Premium
Effects of coumarin and 7OH‐coumarin on bcl‐2 and Bax expression in two human lung cancer cell lines in vitro
Author(s) -
ElinosBáez C.M.,
León F.,
Santos E.
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.04.003
Subject(s) - coumarin , cell culture , in vitro , apoptosis , viability assay , mtt assay , blot , microbiology and biotechnology , cell growth , cell , chemistry , biology , biochemistry , gene , genetics , organic chemistry
Coumarin and its derivative 7‐hydroxycoumarin (7‐OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl‐2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu‐1. The cells were cultured in vitro for 24 h in RPMI 1640 with 1.5% (v/v) ethanol, 1.0 mM ethanolic coumarin or 1.0 mM ethanolic 7‐OHC. Viability was determined in each cell line by an MTT assay. Total protein was extracted from cell lysates and the bcl‐2 and Bax oncoproteins were identified. Western blotting showed a decrease in bcl‐2 and an increase in Bax in A427 cells cultured with coumarin or 7‐OHC. Neither drug changed bcl‐2 expression in Calu‐1 cells compared to solvent controls, and Bax expression was only slightly increased by coumarin. We conclude that 7‐OHC is a more potent inhibitor of cell proliferation than coumarin and has more marked effects on oncoprotein expression. Also, the A427 cell line was more sensitive to the drugs than Calu‐1.