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FMS *Calciumfluor specifically increases mRNA levels and induces signaling via MAPK 42,44 and not FAK in differentiating rat osteoblasts
Author(s) -
Manduca P.,
Marchisio S.,
Astigiano S.,
Zanotti S.,
Galmozzi F.,
Palermo C.,
Palmieri D.
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.03.022
Subject(s) - mapk/erk pathway , osteocalcin , microbiology and biotechnology , alkaline phosphatase , messenger rna , signal transduction , osteoblast , kinase , runx2 , integrin , chemistry , biglycan , biology , medicine , endocrinology , in vitro , gene , extracellular matrix , receptor , biochemistry , enzyme , proteoglycan , decorin
The homeopathic compound of resonance FMS*Calciumfluor (FMS*) reportedly promotes osteogenic differentiation of rat pre‐osteoblasts in vitro. Here, we show that the continuous exposure of differentiating rat osteogenic cells (ROB) to FMS* modulates the level of expression of mRNAs for 7 of the 8 osteogenic markers tested. Alkaline phosphatase (AP), osteocalcin (OC), metalloproteinases (MMP‐2 and −14), procollagenase C (BMP‐1), biglycan (BG) and integrin 1 are expressed at higher levels in FMS*‐treated osteoblasts than in control cultures. MMP‐2 and −14 mRNA are not down‐modulated at mineralization. Also, the pattern of expression induced by FMS* for some of these genes (BMP‐1, BG and integrin 1) is changed, but collagen type I (Coll I) mRNA levels are not affected by treatment with FMS*. This suggests that FMS* modulates mRNA levels and that this is not generalized, but gene(s) specific. We also report that exposure to FMS* rapidly and transiently induces activation of mitogen‐activated protein kinases (MAPKs) 42,44 in populations of early osteoblasts, but not in pre‐osteoblasts, with a cell differentiation stage‐dependent and pertussis toxin (PTX)‐sensitive response. Subsequent to FMS* MAPK signaling activation, an increase in AP and MMP‐14 mRNA is detected, which is also inhibited by PTX, suggesting that FMS* activation of MAPK signaling could be an early event required for the induction of these genes. Exposure to FMS* does not cause changes in the activity of p125 (FAK)‐mediated signaling.