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In vitro hematopoietic differentiation of human embryonic stem cells induced by co‐culture with human bone marrow stromal cells and low dose cytokines
Author(s) -
Wang Jian,
Zhao HuiPing,
Lin Ge,
Xie ChangQing,
Nie DongSong,
Wang QiRu,
Lu GuangXiu
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.03.019
Subject(s) - hemangioblast , stem cell factor , embryoid body , stem cell , haematopoiesis , stromal cell , cd34 , biology , bone marrow , microbiology and biotechnology , embryonic stem cell , endothelial stem cell , hematopoietic stem cell , immunology , adult stem cell , cancer research , in vitro , genetics , gene
Human embryonic stem (hES) cells randomly differentiate into multiple cell types during embryoid body (EB) development and limited studies have focused on directed hematopoietic differentiation. Here, we report that the treatment of hES cells during EBs development with a combination of low dose hematopoietic cytokines, including stem cell factor (SCF), Flt‐3 ligand, vascular endothelial growth factor (VEGF) and human bone marrow stromal cells (hBMSCs), generated cell clusters that contained 8.81% KDR‐positive hemangioblasts, 9.94% CD34‐positive hematopoietic stem cells and 25.7% CD45‐positive mature hematopoietic cells, and expressed hematopoietic genes such as KDR, stem cell leukemia (scl) and runt‐related transcription factor 1 (Runx1). We provide the first evidence for the role of the cytokine—hBMSCs combination in promoting hematopoietic differentiation of hES cells, and thus provide the potential for generation of hematopoietic cells, as well as for understanding early developmental events that govern the initiation of hematopoiesis in humans.