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Proliferative feeder cells support prolonged expansion of human embryonic stem cells
Author(s) -
Xie ChangQing,
Lin Ge,
Yuan Ding,
Wang Jian,
Liu TianCheng,
Lu GuangXiu
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.03.015
Subject(s) - embryonic stem cell , microbiology and biotechnology , stem cell , biology , cell culture , immunology , gene , genetics
Long‐term proliferation of human embryonic stem (hES) cells is currently achieved by co‐culturing with mitotically inactive primary mouse embryonic fibroblasts (mEFs), which serve as feeder cells. This study explores the possibility that proliferative mEFs can be used as feeder cells to maintain the prolonged expansion of hES cells. All undifferentiated hES cell clumps were re‐plated on six different densities of proliferative mEFs. hES colonies cultured on 1 × 10 5 –5 × 10 5 proliferative mEFs amplified over 130 days of continuous culture and remained undifferentiated, as did those cultured on mitotically inactive mEFs. This suggests that certain densities of proliferative mEFs can maintain the propagation of hES cells, which may be helpful for identifying the cytokines and adhesion molecules that are required for their self‐renewal.