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JAK—STAT signaling pathway in pulmonary arterial smooth muscle cells is activated by hypoxia
Author(s) -
Wang GuanSong,
Qian GuiSheng,
Zhou DeShan,
Zhao JiQing
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.03.014
Subject(s) - hypoxia (environmental) , stat3 , stat1 , messenger rna , tyrosine kinase 2 , signal transduction , jak stat signaling pathway , biology , pathogenesis , medicine , endocrinology , receptor , chemistry , microbiology and biotechnology , gene , immunology , tyrosine kinase , biochemistry , growth factor , platelet derived growth factor receptor , organic chemistry , oxygen
Pulmonary arterial smooth muscle cells (PASMC) were divided into a normoxic group (N), 2, 8 and 12 h hypoxic groups (H2, H8 and H12) and an AG490 plus 8 h hypoxic group (AG490). The expression of JAK1, JAK2, JAK3 and TYK2 mRNA was analyzed by reverse transcription‐polymerase chain reaction (RT‐PCR). STAT1 and STAT3 protein expressions were determined by Western blotting. The results showed that the levels of JAK1, JAK2 and JAK3 mRNA did not change significantly in the N group but were increased after 2 h exposure to hypoxia. JAK1, JAK2 and JAK3 mRNA expressions peaked at 8 h. It decreased at 12 h but remained above those in the N group. TYK2 mRNA was not found in either hypoxic or normal PASMC. The phospho‐STAT1 and ‐STAT3 protein levels increased after 2 h exposure to hypoxia. They were about 2.8 and 4.1 times the N group, respectively, after 8 h. They decreased at 12 h but remained above those in the N group. AG490 inhibited phospho‐STAT3 protein expression by about 25.5% at 8 h but did not block the expression of phospho‐STAT1 protein. These findings suggest that hypoxia induces the expression of JAK1, JAK2, JAK3 and phospho‐STAT1 and ‐STAT3 in PASMC. Hypoxia activates the JAKs—STATs signaling pathway, which may participate in the pathogenesis of hypoxic PASMC injury.