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Identification and expression of the amphioxus Cks1 gene
Author(s) -
Yang Hui,
Chen ZhongKe,
Zhang XiaoHui,
Huang XiangWei,
Zhang PeiJun,
Zhang HongWei
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.03.012
Subject(s) - biology , gene , in situ hybridization , vertebrate , phylogenetic tree , phylogenetics , gene expression , evolutionary biology , genetics , sequence analysis , microbiology and biotechnology
A full‐length Cks1 homologue gene, AmphiCks1 , was identified in amphioxus, Branchiostoma belcheri tsingtauense . Sequence characteristics, phylogeny and patterns of expression during embryonic and larval development were established. The protein predicted from AmphiCks1 showed high sequence identity with vertebrate and invertebrate homologues. Protein structural studies and phylogenetic analysis suggested that Cks homologues are evolutionarily conserved. The AmphiCks1 transcript was detected in most early developmental stages by northern blotting and whole‐mount in situ hybridization, suggesting a role for the gene in cell division.