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Down‐regulation of immediate early gene egr‐1 expression in rat C6 glioma cells by short‐term exposure to high salt culture medium
Author(s) -
Morita Kyoji,
Arimochi Hideki,
Yoshida Shigeru
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.01.002
Subject(s) - gene expression , messenger rna , salt (chemistry) , monensin , cell culture , chemistry , intracellular , sucrose , veratridine , microbiology and biotechnology , biology , gene , sodium , biochemistry , sodium channel , genetics , organic chemistry
Influence of high salt culture conditions on the expression of immediate early gene egr‐1 in rat C6 glioma cells was investigated by measuring both Egr‐1 mRNA and protein levels in the cells exposed to the medium containing high concentrations of NaCl. The exposure to high salt medium reduced Egr‐1 mRNA and protein levels, while Egr‐1 mRNA levels were not altered by the medium containing either sucrose or glycerol. Veratridine and monensin also reduced Egr‐1 mRNA levels, similar in extent to that induced by high salt medium. Imaging analysis indicated that the exposure to high salt medium induced the elevation of Na + levels within the cells. These results indicate that neither hyperosmotic pressure nor ionic strength of high salt medium contribute to the reduction of Egr‐1 expression, and suggest that the elevation of intracellular Na + concentration is closely associated with the down‐regulation of egr‐1 gene expression.