Premium
Apoptosis effects of Xrel3 c‐Rel/Nuclear Factor‐kappa B homolog in human cervical cancer cells
Author(s) -
Shehata Marlene,
Shehata Marian,
Shehata Fady,
Pater Alan
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.12.014
Subject(s) - apoptosis , hela , cisplatin , biology , cancer research , microbiology and biotechnology , cervical cancer , cell cycle , cancer cell , cancer , cell , chemotherapy , genetics
Cervical cancer is considered a common yet preventable cause of death in women. In this report, we studied the role of the NF‐κB gene family in HeLa human cervical cancer cells, using the Xrel3 c‐Rel homologue of Xenopus laevis . The expression of Xrel3/c‐Rel slowed cell growth 6‐fold, consistent with an upregulated expression of the cell cycle inhibitor p21. The activated PARP apoptosis effector was significantly increased (P<0.01). Based on cell viability assays Xrel3 provided an anti‐apoptotic effect in 1 μM cisplatin, and this was associated with significantly lower levels of the apoptotic proteins Bax and MDM‐2 (P<0.05). Furthermore, there was a 3‐fold drop in the level of the tumor suppressor protein p53. In 5 μM cisplatin, expression of HeLa Xrel3 enhanced apoptosis by significantly increasing the expression of the apoptotic proteins Bax and MDM‐2 (P<0.05). However, the tumor suppressor protein p53 showed a significant decrease (P<0.05) relative to the control. Thus, c‐Rel/NF‐κB may potentially be of clinical significance, especially in tumors exhibiting resistance to high‐level chemotherapy.