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Suppression of macrophage infiltration into the conjunctiva by clodronate liposomes in experimental immune‐mediated blepharoconjunctivitis
Author(s) -
Fukushima Atsuki,
Ozaki Akemi,
Ishida Waka,
Rooijen Nico,
Fukata Kazuyo,
Ueno Hisayuki
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.12.011
Subject(s) - conjunctiva , infiltration (hvac) , spleen , immune system , pathology , macrophage , medicine , immunology , chemistry , biochemistry , in vitro , physics , thermodynamics
Macrophages infiltrate the conjunctiva in severe cases of allergic conjunctivitis (AC) such as atopic keratoconjunctivitis (AKC). We established experimental immune‐mediated blepharoconjunctivitis (EC) in Brown Norway (BN) rats as a model for severe types of AC. We investigated whether macrophage infiltration in the conjunctiva in this EC model is inhibited by clodronate liposomes (CL 2 MDP‐lip). The numbers of ED1‐positive but not ED2‐positive macrophages in the conjunctivas were increased by the induction of EC. Subconjunctival injection of CL 2 MDP‐lip decreased the number of ED2‐positive but not ED1‐positive macrophages in the conjunctivas of naive rats. CL 2 MDP‐lip did not affect macrophages in the spleen. Subconjunctival injection of CL 2 MDP‐lip into EC‐developing BN rats decreased the number of ED2‐positive macrophages at all the time points. ED1‐positive cell infiltration was inhibited when treatment was administered just prior to OVA challenge. Intravenous injection of CL 2 MDP‐lip decreased the number of ED2‐positive cells in the conjunctiva. Thus, we conclude that CL 2 MDP‐lip inhibits infiltration of macrophages into the conjunctiva within 24 h of antigen challenge.