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HLA‐G in murine peripheral blood after interruption of pregnancy
Author(s) -
Dendrinos S.,
Kalabalikis G.,
Makrakis E.,
Papasteriades C.,
Creatsas G.,
Katsorchis T.
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.12.005
Subject(s) - pregnancy , hla g , human leukocyte antigen , peripheral blood , andrology , medicine , mifepristone , transgene , monoclonal antibody , endocrinology , immunology , biology , antigen , antibody , genetics , gene
HLA‐G antigens are highly expressed in maternal peripheral blood during early pregnancy in transgenic mice. In this study, we determined the levels of HLA‐G in white blood cells during early pregnancy and after interruption of pregnancy in triple transgenic mice (H‐2K b /HLA‐G, hβ2m, and hCD2/hCD8‐TRI). The pregnancies were interrupted on day 7 using the anti‐progesterone agent mifepristone (RU486). Blood samples of 20 pregnant TRI mice were taken and the HLA‐G levels were determined on days 2, 4 and 6 of pregnancy and on days 9, 11 and 13 after fertilization. The monoclonal antibody W6/32, specific for monomorphic determinant HLA class I molecules, was used in combination with an immunolocalization method using a photonic microscope. The HLA‐G levels increased gradually on days 2, 4 and 6 of pregnancy, and the interruption of pregnancy on day 7 was followed by a decrease of HLA‐G levels. The data indicate that pregnancy is characterized by the early presence of HLA‐G in maternal peripheral blood in TRI transgenic mice and suggest that HLA‐G may serve as a useful indicator for pregnancy maintenance and well‐being.