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Production of transgenetic sugarbeet ( Beta vulgaris L.) plants resistant to phosphinothricin
Author(s) -
Kishchenko E.M.,
Komarnitskii I.K.,
Kuchuk N.V.
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.11.003
Subject(s) - beta (programming language) , biology , botany , horticulture , computer science , programming language
A method of Agrobacterium‐mediated genetic transformation of sugarbeet ( Beta vulgaris L.) with vacuum infiltration has been developed. Aseptic 3‐weeks old etiolated seedlings of two diploid O‐type sugarbeet lines (KS3 and KS7) have been used for genetic transformation. Transgenic sugarbeet plants carrying the reporter β‐glucuronidase gene have been selected for their resistance to glufosinate ammonium herbicide. Integration of transgenes into sugarbeet genome was confirmed with GUS assay and PCR using primers for bar and gusA genes.