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Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture‐induced rearrangements and interspecies polymorphism
Author(s) -
Andreev I.O.,
Spiridonova K.V.,
Solovyan V.T.,
Kunakh V.A.
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.11.002
Subject(s) - biology , ribosomal rna , gene , polymorphism (computer science) , genetics , rna , parallelism (grammar) , genotype , linguistics , philosophy
An analysis of 18S–25S and 5S rRNA genes in intact plants and cultured tissues of some Rauwolfia species was performed to compare these sequences variability occurred as a result of the species evolution in nature and that induced by tissue culture. The restriction fragment length polymorphism of 18S–25S and 5S rDNA was found both in intact plants of various Rauwolfia species and in long‐term Rauwolfia serpentina tissue cultures. In addition, changes in the amount of 18S–25S rRNA genes were observed in long‐term R. serpentina tissue cultures. The results demonstrate that rDNA variability observed in intact plants as well as in long‐term cultures is attributed to differences in the same regions of ribosomal RNA genes.