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The influence of 2‐chlorodeoxyadenosine (2‐CdA) on the adenine energy charge and glutathione content of human erythrocytes
Author(s) -
Marczak A.,
Bukowska B.,
Koczmara M.,
Jóźwiak Z.
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.09.009
Subject(s) - echinocyte , glutathione , energy charge , incubation , chemistry , biochemistry , cladribine , biophysics , red blood cell , biology , enzyme , adenylate kinase , genetics
We have examined the effect of exposure of human erythrocytes to the new chemotherapy drug 2‐chlorodeoxyadenosine (2‐CdA, cladribine), focusing on the glutathione (GSH and GSSG) content and the adenine energy charge (AEC). Incubation of erythrocytes with 0.1–5 μg/ml 2‐CdA induced no significant change in the reduced or total glutathione level or in the AMP and ATP concentrations. The ADP concentration increased slightly and the AEC value is in the range typical of healthy organisms. Incubation of erythrocytes with 2‐CdA also caused cell shape changes, converting most of the cells to echinocytes.