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Subnucleolar distribution and organization of Vicia faba L. rDNA in situ
Author(s) -
Long Hong,
Sun Haijing,
Zeng Xianlu,
Hao Shui,
Jiao Mingda
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.08.004
Subject(s) - nucleolus , vicia faba , chromatin , dna , biology , ribosomal dna , nucleolus organizer region , ribosomal rna , staining , microbiology and biotechnology , in situ , gene , botany , genetics , chemistry , nucleus , organic chemistry , phylogenetics
The distribution and organization of nucleolar DNA in Vicia faba L. was analyzed by specific cytochemical staining using NAMA‐Ur. The results showed that nucleolar DNA was distributed in the FCs and at the FC/DFC junctions. Statistical analysis showed that the rRNA genes occupied about one‐third of the total dense fibrillar component region. The rDNA was condensed in some regions and uncondensed in others. Nucleolus‐associated chromatin extended from outside the nucleolus to the periphery of the FCs via nucleolar channels, suggesting a possible origin for nucleolar DNA.