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Influence of cytoplasmic deletions on the filopodia‐inducing effect of syndecan‐3
Author(s) -
Berndt Christine,
Montañez Eloi,
Villena Joan,
Fabre Myriam,
Vilaró Senén,
Reina Manuel
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.08.003
Subject(s) - syndecan 1 , cytoplasm , transmembrane protein , microbiology and biotechnology , filopodia , cell , heparan sulfate , biology , cell membrane , chemistry , biochemistry , receptor
Syndecans, transmembrane heparan sulfate proteoglycans (HSPG), mediate cell—cell and cell—matrix adhesion thereby controlling cell movement and shape. Syndecan cytoplasmic domains are very short (ca. 30 amino acids) and divided into two constant regions (C1 and C2) separated by one variable (V) region. Here we attempted to map the cytoplasmic region responsible for the filopodia‐inducing effect of syndecan‐3. We found that only the C1‐region was necessary for this effect. In addition, the deletion of the C2‐region led to extensive membrane blebbing. Nevertheless, the elimination of the entire cytoplasmic region did not affect delivery of syndecan‐3 to the plasma membrane. These results indicate that the different regions of syndecan‐3 cytoplasmic domain have different functions probably by binding to distinct proteins.