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Identification and characterization of coiled body‐like structures in pea ( Pisum sativum L.)
Author(s) -
Long Hong,
Sun Haijing,
Zeng Xianlu,
Hao Shui,
Jiao Mingda
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.08.002
Subject(s) - fibrillarin , nucleolus , organelle , biology , immunoelectron microscopy , microbiology and biotechnology , meristem , cajal body , pisum , nucleus , botany , rna , genetics , rna splicing , gene , antibody , shoot
Coiled bodies (CBs) are nuclear organelles which were considered as “universal” nuclear structures in eukaryotic cells, but the formation and function of CBs, especially in plant cells, remained unclear. In this article we reported that CBs in meristematic cells of pea are oval to round obstacles in nucleus and in adjacent to nucleolus, often have the same electron density with nucleolus. We found that CBs could be stained by the rRNP preference staining method, but no rDNA was detected in the structure. Furthermore, our results of immunoelectron microscopy showed that several processing factors, include fibrillarin, U3 snoRNA and ITS1, were present in CB. It seems probable that CBs is derived structurally from nucleolus and act as transport, storage and processing subnucleolar organelles.