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Expression and regulation of interleukin‐23 subunits in human peripheral blood mononuclear cells and hematopoietic cell lines in response to various inducers
Author(s) -
Ma XiaoTong,
Zhang XiuJun,
Zhang Bin,
Geng YiQi,
Lin YongMin,
Li Ge,
Wu KeFu
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.07.002
Subject(s) - peripheral blood mononuclear cell , haematopoiesis , inducer , peripheral blood , immunology , biology , microbiology and biotechnology , stem cell , in vitro , gene , genetics
IL‐23 is a novel cytokine composed of an IL‐12 p40 and a p19 subunit. We analyzed human peripheral blood mononuclear cells (PBMC) and hematopoietic cell lines for constitutive expression of IL‐23 and IL‐12 subunits and expression following exposure to various stimuli, and investigated the mechanisms of their induction by LPS and SAC. IL‐23 p19 and IL‐12 p35 mRNAs were expressed in fresh PBMC, and expression of all IL‐23 and IL‐12 subunits was up‐regulated by LPS and SAC. LPS‐induced increase of IL‐23 and IL‐12 subunits expression was CD14‐dependent, while CD14 was not involved in SAC‐induced p19 transcription. Both LPS‐ and SAC‐induced subunits expression required p38 MAPK pathway. PHA effected an increase of p19 mRNA in both CD4 + and CD8 + T cells, whereas p35 was minimally regulated by PHA. IFN‐γ primed monocytes for LPS stimulation of p19, p35 and p40 expression. p19 mRNA was detectable in most hematopoietic cell lines tested but p35 distribution was more restricted. A phorbol diester enhanced p19, p35 and p40 expression in EBV‐positive cell lines. Our results suggest that both the subunits of IL‐23 are tightly regulated; the expression pattern and regulation mode of IL‐23 p19 is similar to as well as distinct from that of IL‐12 p35.