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Expression of polypeptide GalNAc‐transferases in hematopoietic stem/progenitor cells
Author(s) -
Cheng Lamei,
Wang Xianyou,
Tan Mengqun,
Zhou Xiaoying,
Wang Qiru
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.06.001
Subject(s) - haematopoiesis , progenitor cell , stem cell , microbiology and biotechnology , biology , computational biology
The members of the UDP‐GalNAc:polypeptide N ‐acetylgalactosaminyltransferase (pp‐GalNAc‐T) family, which transfer GalNAc to polypeptide serine and threonine residues, initiate mucin‐type O‐linked glycosylation. There are at least 13 functionally characterized members of this family in humans, but no studies have been reported of pp‐GalNAc‐T isoforms in hematopoietic cells. We isolated and purified CD34 + hematopoietic cells from adult bone marrow by magnetic cell sorting and induced them to differentiate into megakaryocytic lineage cells using an optimal combination of hematopoietic growth factors in serum‐free liquid medium. RT‐PCR revealed that CD34 + cells expressed pp‐GalNAc‐T1, T2, T3, T4, T6, T7, T10, T11 and T14, but not pp‐GalNAc‐T8, T9, T12 and T13. The megakaryocytic lineage cells showed significant increases in the expression of pp‐GalNAc‐T3, T8, T9, T10 and T13, but pp‐GalNAc‐T11 and T14 became undetectable. In summary, many pp‐GalNAc‐T isoforms were expressed in CD34 + cells but the expression pattern changed during differentiation into megakaryocytes. The expression patterns of pp‐GalNAc‐Ts may be necessary to ensure proper O ‐glycosylation of mucin‐type proteins expressed in CD34 + and megakaryocytic cells.