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Prooxidative effects of TEMPO on human erythrocytes
Author(s) -
Balcerczyk Aneta,
Łuczak Katarzyna,
Soszyński Mirosław,
Bartosz Grzegorz
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.04.014
Subject(s) - ferricyanide , methemoglobin , glutathione , extracellular , intracellular , chemistry , hemoglobin , incubation , red blood cell , biochemistry , potassium ferricyanide , biophysics , biology , inorganic chemistry , enzyme
The prooxidative effects of 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO) were observed in human erythrocytes. Incubation of red blood cells with the membrane‐permeable TEMPO leads to a decrease in the concentration of intracellular reduced glutathione, accompanied by the reduction of TEMPO. Extracellular ferricyanide inhibited the loss of glutathione and reduction of TEMPO. TEMPO induced glutathione release from the cells and oxidation of hemoglobin to methemoglobin; ferricyanide prevented these effects. These results indicate that TEMPO may act as an oxidant to erythrocytes, whilst extracellular ferricyanide protects against its effects.