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Cytotoxicity of tamoxifen in normal and tumoral cell lines and its ability to induce cellular transformation in vitro
Author(s) -
Petinari Leandro,
Kohn Luciana Konecny,
Carvalho João Ernesto,
Genari Selma Candelária
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.04.008
Subject(s) - tamoxifen , in vitro , cytotoxicity , transformation (genetics) , cancer research , chemistry , microbiology and biotechnology , cell culture , pharmacology , biology , medicine , cancer , biochemistry , breast cancer , genetics , gene
Abstract Tamoxifen (TAM) is a non‐steroidal anti‐estrogen used to treat patients with estrogen receptor‐positive breast cancer and as a chemopreventive agent against breast cancer in high risk pre‐ and post‐menopausal women. However, recent studies have shown that tamoxifen causes endometrial and hepatic cancer. In this study, we examined the effects of tamoxifen (5, 10, 25 and 50 μM) on the growth and proliferation of nine tumoral cell lines (UACC62, MCF‐7, NCI‐460, K‐562, OVCAR‐03, PC‐03, HT‐29, 786‐0, NCI‐ADR) and non‐tumoral cell lines (3T3, V79, MDCK, VERO). Chinese hamster lung fibroblasts (V79) were the most sensitive lineage to tamoxifen, with 21.6% of the cells showing apoptosis at 50 μM TAM. Microscopic analysis showed that, the cellular transformation caused by TAM in V79 cells was similar to that seen with 7,12‐dimethylbenz(a)anthracene, thus indicating the carcinogenicity of TAM.