Premium
Enrichment and characterization of mouse putative epidermal stem cells
Author(s) -
Zhou Jiaxi,
Jia Liwei,
Yang Yongjun,
Peng Sha,
Cao Yujing,
Duan Enkui
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.04.007
Subject(s) - stem cell , biology , cell sorting , stem cell marker , microbiology and biotechnology , adult stem cell , propidium iodide , neural stem cell , cancer stem cell , population , neurosphere , in vitro , endothelial stem cell , flow cytometry , biochemistry , apoptosis , demography , programmed cell death , sociology
Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal stem cells and culturing them has been challenging. In this study, putative epidermal stem cells of mouse were isolated by combining Hoechst 33342 and propidium iodide staining with fluorescence‐activated cell sorting. Molecular markers expression pattern analysis showed that cytokeratin 14, integrin β 1 and p63 are expressed in the sorted putative stem cells, but not active β‐catenin, nestin and involucrin. Our results provide further supporting data that mouse putative epidermal stem cells could be successfully isolated by combining Hoechst dye staining with fluorescence‐activated cell sorting and cultured in vitro. The cultured mouse putative epidermal stem cells could be used as a potent tool for studying stem cell biology and testing stem cell therapy.