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Presence and localization of epidermal growth factor (EGF)‐ and EGF‐receptor‐like immunoreactivity in Tetrahymena
Author(s) -
Csaba G.,
Kovács P.,
Pállinger Éva
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.04.002
Subject(s) - tetrahymena , epidermal growth factor , biology , flow cytometry , receptor , microbiology and biotechnology , monoclonal antibody , confocal microscopy , epidermal growth factor receptor , medicine , antibody , biochemistry , immunology
The presence of EGF‐ and EGF‐receptor‐like immunoreactivity in Tetrahymena was studied with the help of FITC‐labelled monoclonal antibodies, using flow cytometry and confocal microscopy. Tetrahymena has endogeneous EGF and treatment with it significantly increases the hormone content of the cells. The hormone is diffusely localized, particularly in the cytopharynx, where it forms a structure larger than 10 μm. EGF‐receptors are also demonstrable, particularly in the cortical region in connection with cilia, and EGF treatment significantly enhances cortical fluorescence. The relationships between these observations and literary data on the effects of EGF in Tetrahymena are discussed.