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A small heat‐shock protein, p26, from the crustacean Artemia protects mammalian cells (Cos‐1) against oxidative damage
Author(s) -
Collins Crista H.,
Clegg James S.
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.03.014
Subject(s) - heat shock protein , oxidative phosphorylation , oxidative damage , crustacean , microbiology and biotechnology , shock (circulatory) , hsp70 , biology , chemistry , oxidative stress , biochemistry , zoology , medicine , gene
A small heat‐shock protein (p26) purified from stress‐resistant embryos of the crustacean, Artemia franciscana , was introduced into cultured cells of green monkey kidney (Cos‐1) using the BioPORTER® delivery system. Cells containing p26 exhibited impressive resistance to hydrogen peroxide compared to controls. Introduction of the disaccharide trehalose did not provide protection against oxidative damage, but enhanced substantially the protective performance of p26 when both were present. These studies extend previous research on the protective role played by p26 in cells exposed to various forms of stress, presumably through its ability to function as a molecular chaperone.