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Low concentration of oxidant and nitric oxide donors stimulate proliferation of human endothelial cells in vitro
Author(s) -
Łuczak Katarzyna,
Balcerczyk Aneta,
Soszyński Mirosław,
Bartosz Grzegorz
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.03.004
Subject(s) - sodium nitroprusside , catalase , nitric oxide , umbilical vein , superoxide dismutase , reactive oxygen species , hydrogen peroxide , chemistry , stimulation , superoxide , cell growth , in vitro , endothelial stem cell , menadione , biochemistry , sodium ascorbate , microbiology and biotechnology , oxidative stress , biology , enzyme , endocrinology , ascorbic acid , food science , organic chemistry
Proliferation of human umbilical vein endothelial cells in vitro was inhibited by high concentrations of oxidants and nitric oxide donors but stimulated by low (micromolar or submicromolar) concentrations of hydrogen peroxide, menadione, tert ‐butyl hydroperoxide, AAPH, nitroglycerin, SIN‐1 and sodium nitroprusside. The stimulation seems to be dependent upon generation of secondary reactive oxygen species as inferred from attenuation of cell proliferation by superoxide dismutase and catalase. These results point to another type of possible artefact of cell culture, viz. stimulation of cell proliferation by low concentrations of oxidants.