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Modification of intracellular free calcium in cultured F2408 embryo fibroblasts by 3‐substituted‐2‐thiohydantoin derivatives
Author(s) -
Incesu Z.,
Benkli K.,
Akalin G.,
Kaplancikli Z.A.
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.01.003
Subject(s) - formazan , cytotoxicity , intracellular , chemistry , in vitro , calcium , embryo , calcium in biology , fibroblast , cytotoxic t cell , ic50 , mtt assay , incubation , biochemistry , microbiology and biotechnology , stereochemistry , biology , organic chemistry
3‐substituted‐2‐thiohydantoin derivatives were synthesized and their structures elucidated by IR, 1 H‐NMR spectroscopy and elemental analysis. The cytotoxicity of the 2‐thiohydantoin derivatives to rat embryo fibroblasts (F2408) in vitro was determined, and the effects of these compounds on intracellular free Ca 2+ , [Ca 2+ ] i , were measured by spectrofluorophotometry. Cytotoxicity was determined by metabolic reduction of a tetrazolium salt to a formazan dye (MTT assay). Compounds 4 and 7 showed cytotoxic activity, with IC 50 values in the range of 1–1.2 μM. Introduction of either chlorophenyl, metoxyphenyl, nitrophenyl or benzyl groups at C‐3 resulted in concentration‐dependent cytotoxic effects. Compounds 1–6 at 1 μM or more significantly increased [Ca 2+ ] i in a dose‐dependent manner in the cultured fibroblasts. This action may have been mediated through intracellular calcium stores.