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Effects of triethylene tetraamine on telomerase activity and proliferation in HeLa cells
Author(s) -
Yin Fei,
Liu Jianhui,
Peng Xiaojun
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2004.01.001
Subject(s) - hela , telomerase , telomere , cell cycle , flow cytometry , cell growth , microbiology and biotechnology , biology , cell , cell culture , chemistry , biochemistry , dna , genetics , gene
Telomerase, which is required to maintain telomeres, has attracted considerable attention as a target for anticancer therapy. In this study, we investigated the inhibition of HeLa cell telomerase activity and cell cycle progression by triethylene tetraamine (TETA), using a modified telomeric repeat amplification protocol (TRAP) assay, and flow cytometry. TETA inhibited telomerase activity in HeLa cell extracts, with an IC 50 of about 7.8 μM. Coupled with this inhibition, TETA also increased the proportion of cells in the G 1 phase of the cell cycle in a dose‐dependent manner. These findings demonstrate that TETA is a potent inhibitor of telomerase in micromolar concentrations, and inhibits the proliferation of HeLa cells by arresting them in G 1 .