Premium
Cultured peritoneal mesothelial cells exhibit apical primary cilia
Author(s) -
Bird Stephen D.,
Legge Michael,
Walker Robert J
Publication year - 2004
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2003.11.014
Subject(s) - cilium , microbiology and biotechnology , immunocytochemistry , cysteine , chemistry , biology , biochemistry , endocrinology , enzyme
This study examined primary cilia on cultured human and rabbit peritoneal mesothelial cells (PMC) and investigated factors that influence ciliary expression. Primary cilia were examined with indirect immunocytochemistry, laser scanning confocal microscopy and scanning electron microscopy. Ciliary expression was evaluated in cultures with or without l ‐cysteine (0.25 mM) or exposure to Ca 2+ ‐free Krebs—Ringer solution supplemented with EGTA, 0.5 mM. This treatment disrupted cell monolayer integrity. Cilia were counted and normalized to total cell counts using NIH image. Primary cilia were identified on both human and rabbit PMC. Cells treated with l ‐cysteine expressed significantly more cilia compared with monolayers deprived of l ‐cysteine. Exposure to Ca 2+ ‐free Krebs—Ringer solution significantly reduced cilia (5.9±1.0%, n =7). Although ciliary expression could be augmented with l ‐cysteine, approximately 60% of human PMC and 84% of rabbit PMC did not exhibit cilia. Together, these data show that monolayers of PMC express apical cilia that can be augmented with l ‐cysteine, independently of increased cell density.