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Upregulation of cyp2e1 and cyp3a activities in histamine‐deficient histidine decarboxylase gene targeted mice
Author(s) -
Tamási Viola,
Fülöp A. Kristóf,
Hegyi Krisztina,
Monostory Katalin,
Falus András
Publication year - 2003
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2003.09.006
Subject(s) - cyp3a , cyp2e1 , histidine decarboxylase , histamine , chlorzoxazone , chemistry , aromatic l amino acid decarboxylase , pharmacology , biochemistry , cytochrome p450 , histidine , microbiology and biotechnology , biology , enzyme
Histamine is a biogenic amine with multiple physiological functions. Its importance in allergic inflammation is well characterized; moreover, it plays a role in the regulation of gastric acid production, various hypothalamic functions, such as food uptake, and enhancing TH2 balance during immune responses. Using histidine decarboxylase gene targeted (HDC −/− ) BALB/c mice, we studied the effect of the absence of histamine on four cytochrome P450 enzyme activities. Their selective substrates were measured: ethoxyresorufin O ‐dealkylase activity of CYP1A, pentoxyresorufin O ‐dealkylase activity of CYP2B, chlorzoxazone 6‐hydroxylase activity of CYP2E1 and ethylmorphine N ‐demethylase activity of CYP3A. The results indicate a significant elevation of CYP2E1 and CYP3A activities, however, no change in CYP1A and CYP2B activities was seen in HDC targeted mice compared to wild type controls with identical genetic backgrounds.