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Expression of cell adhesion molecules in the adriamycin‐induced esophageal atresia rat model
Author(s) -
Tugay Melih,
Filiz Serdar,
Dalçik Hakki,
Güvenç B. Haluk,
Dalçik Cannur,
Korkmaz Mevlit,
Sozubir Selami
Publication year - 2003
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2003.08.002
Subject(s) - atresia , immunostaining , cell adhesion molecule , cadherin , embryo , biology , cell , adhesion , cell adhesion , microbiology and biotechnology , embryonic stem cell , esophagus , andrology , pathology , immunohistochemistry , medicine , anatomy , chemistry , immunology , biochemistry , organic chemistry , gene
Cell adhesion molecules are well‐known membrane glycoproteins widely expressed during embryonic development that play a crucial role in cell division, migration and differentiation. We investigated the cell—matrix relationship using N‐CAM and pan‐cadherin adhesion molecules in the adriamycin‐induced esophageal atresia (EA) rat model in the hope of finding a clue to the mechanisms of this unique anomaly. Time‐mated pregnant Sprague—Dawley rats were given either saline or adriamycin on days 8 and 9 of gestation. Embryos were harvested on the 18th day of gestation. Esophageal specimens obtained from adriamycin‐exposed embryos with (EA+) or without esophageal atresia (EA−) and from saline‐exposed embryos were immunostained with N‐CAM and pan‐cadherin primary antisera. The esophageal specimens from control and EA− groups revealed similar immunostaining properties: weak N‐CAM and pan‐cadherin immunoreactivity. In contrast, the EA+ group showed intense immunoreactivity. Our study demonstrated an increased synthesis of N‐CAM and pan‐cadherin in the epithelial cells of the atretic esophagus and trachea. These results suggest that embryonic cell—cell and cell—matrix interactions may play a crucial role in the development of adriamycin‐induced EA.