TCR+/BCR+ dual-expressing cells and their associated public BCR clonotype are not enriched in type 1 diabetes | Zendy

Alberto Sada Japp | Zendy; Wenzhao Meng | Zendy; Aaron M. Rosenfeld | Zendy; Daniel J. Perry | Zendy; Puchong Thirawatanad | Zendy; Rhonda Bacher | Zendy; Chengyang Liu | Zendy; Jay Gardner | Zendy; Mark A. Atkinson | Zendy; Klaus H. Kaestner | Zendy; Todd M. Brusko | Zendy; Ali Naji | Zendy; Eline T. Luning Prak | Zendy; Michael R. Betts | Zendy

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TCR+/BCR+ dual-expressing cells and their associated public BCR clonotype are not enriched in type 1 diabetes

Author(s) -

Alberto Sada Japp,

Wenzhao Meng,

Aaron M. Rosenfeld,

Daniel J. Perry,

Puchong Thirawatanad,

Rhonda Bacher,

Chengyang Liu,

Jay Gardner,

Mark A. Atkinson,

Klaus H. Kaestner,

Todd M. Brusko,

Ali Naji,

Eline T. Luning Prak,

Michael R. Betts

Publication year - 2021

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2020.11.035

Subject(s) - biology , clone (java method) , t cell receptor , flow cytometry , breakpoint cluster region , b cell receptor , b cell , immunology , t cell , microbiology and biotechnology , receptor , gene , genetics , immune system , antibody

Ahmed and colleagues recently described a novel hybrid lymphocyte expressing both a B and T cell receptor, termed double expresser (DE) cells. DE cells in blood of type 1 diabetes (T1D) subjects were present at increased numbers and enriched for a public B cell clonotype. Here, we attempted to reproduce these findings. While we could identify DE cells by flow cytometry, we found no association between DE cell frequency and T1D status. We were unable to identify the reported public B cell clone, or any similar clone, in bulk B cells or sorted DE cells from T1D subjects or controls. We also did not observe increased usage of the public clone VH or DH genes in B cells or in sorted DE cells. Taken together, our findings suggest that DE cells and their alleged public clonotype are not enriched in T1D. This Matters Arising paper is in response to Ahmed et al. (2019), published in Cell. See also the response by Ahmed et al. (2021), published in this issue.

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