Molecular Architecture of the SARS-CoV-2 Virus | Zendy

HangPing Yao | Zendy; Yutong Song | Zendy; Yong Chen | Zendy; Nanping Wu | Zendy; Jialu Xu | Zendy; Chujie Sun | Zendy; Jiaxing Zhang | Zendy; Tian-Hao Weng | Zendy; Zheyuan Zhang | Zendy; Zhigang Wu | Zendy; Linfang Cheng | Zendy; Danrong Shi | Zendy; Xiangyun Lu | Zendy; Jianlin Lei | Zendy; Max Crispin | Zendy; Yigong Shi | Zendy; Lanjuan Li | Zendy; Sai Li | Zendy

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Molecular Architecture of the SARS-CoV-2 Virus

Author(s) -

HangPing Yao,

Yutong Song,

Yong Chen,

Nanping Wu,

Jialu Xu,

Chujie Sun,

Jiaxing Zhang,

Tian-Hao Weng,

Zheyuan Zhang,

Zhigang Wu,

Linfang Cheng,

Danrong Shi,

Xiangyun Lu,

Jianlin Lei,

Max Crispin,

Yigong Shi,

Lanjuan Li,

Sai Li

Publication year - 2020

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2020.09.018

Subject(s) - biology , virus , glycan , virology , ribonucleoprotein , recombinant dna , glycoprotein , rna , viral envelope , covid-19 , microbiology and biotechnology , biochemistry , gene , medicine , disease , pathology , infectious disease (medical specialty)

SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA). Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å. Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans. The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed. Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.

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