Open AccessFate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells
Author(s) -
Zhaoyuan Liu,
Yaqi Gu,
Svetoslav Chakarov,
Camille Blériot,
Immanuel Kwok,
Xin Chen,
Amanda Shin,
Weijie Huang,
Regine J. Dress,
CharlesAntoine Dutertre,
Andreas Schlitzer,
Jinmiao Chen,
Lai Guan Ng,
Honglin Wang,
Zhiduo Liu,
Bing Su,
Florent Ginhoux
Publication year - 2019
Publication title -
cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 26.304
H-Index - 776
eISSN - 1097-4172
pISSN - 0092-8674
DOI - 10.1016/j.cell.2019.08.009
Subject(s) - biology , monocyte , haematopoiesis , fate mapping , progenitor cell , inflammation , microbiology and biotechnology , embryonic stem cell , homeostasis , granulocyte , immunology , bone marrow , macrophage , stem cell , gene , genetics , in vitro
Most tissue-resident macrophage (RTM) populations are seeded by waves of embryonic hematopoiesis and are self-maintained independently of a bone marrow contribution during adulthood. A proportion of RTMs, however, is constantly replaced by blood monocytes, and their functions compared to embryonic RTMs remain unclear. The kinetics and extent of the contribution of circulating monocytes to RTM replacement during homeostasis, inflammation, and disease are highly debated. Here, we identified Ms4a3 as a specific gene expressed by granulocyte-monocyte progenitors (GMPs) and subsequently generated Ms4a3 TdT reporter, Ms4a3 Cre , and Ms4a3 CreERT2 fate-mapping models. These models traced efficiently monocytes and granulocytes, but no lymphocytes or tissue dendritic cells. Using these models, we precisely quantified the contribution of monocytes to the RTM pool during homeostasis and inflammation. The unambiguous identification of monocyte-derived cells will permit future studies of their function under any condition.
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