Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication | Zendy

Jyoti Batra | Zendy; Judd F. Hultquist | Zendy; Dandan Liu | Zendy; Olena Shtanko | Zendy; John Von Dollen | Zendy; Laura Satkamp | Zendy; Gwendolyn Μ. Jang | Zendy; Priya Luthra | Zendy; Toni M. Schwarz | Zendy; Gabriel I. Small | Zendy; Eusondia Arnett | Zendy; Manu Anantpadma | Zendy; Ann Reyes | Zendy; Daisy W. Leung | Zendy; Robyn M. Kaake | Zendy; Paige Haas | Zendy; Carson B. Schmidt | Zendy; Larry S. Schlesinger | Zendy; Douglas LaCount | Zendy; Robert A. Davey | Zendy; Gaya K. Amarasinghe | Zendy; Christopher F. Basler | Zendy; Nevan J. Krogan | Zendy

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Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication

Author(s) -

Jyoti Batra,

Judd F. Hultquist,

Dandan Liu,

Olena Shtanko,

John Von Dollen,

Laura Satkamp,

Gwendolyn Μ. Jang,

Priya Luthra,

Toni M. Schwarz,

Gabriel I. Small,

Eusondia Arnett,

Manu Anantpadma,

Ann Reyes,

Daisy W. Leung,

Robyn M. Kaake,

Paige Haas,

Carson B. Schmidt,

Larry S. Schlesinger,

Douglas LaCount,

Robert A. Davey,

Gaya K. Amarasinghe,

Christopher F. Basler,

Nevan J. Krogan

Publication year - 2018

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2018.08.044

Subject(s) - biology , ebola virus , nucleoprotein , virology , viral replication , gene knockdown , transcription (linguistics) , ebolavirus , microbiology and biotechnology , viral protein , virus , cell culture , genetics , philosophy , linguistics

Ebola virus (EBOV) infection often results in fatal illness in humans, yet little is known about how EBOV usurps host pathways during infection. To address this, we used affinity tag-purification mass spectrometry (AP-MS) to generate an EBOV-host protein-protein interaction (PPI) map. We uncovered 194 high-confidence EBOV-human PPIs, including one between the viral transcription regulator VP30 and the host ubiquitin ligase RBBP6. Domain mapping identified a 23 amino acid region within RBBP6 that binds to VP30. A crystal structure of the VP30-RBBP6 peptide complex revealed that RBBP6 mimics the viral nucleoprotein (NP) binding to the same interface of VP30. Knockdown of endogenous RBBP6 stimulated viral transcription and increased EBOV replication, whereas overexpression of either RBBP6 or the peptide strongly inhibited both. These results demonstrate the therapeutic potential of biologics that target this interface and identify additional PPIs that may be leveraged for novel therapeutic strategies.

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