Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation | Zendy

Markus Mund | Zendy; Jan van der Beek | Zendy; J.R. Deschamps | Zendy; Serge Dmitrieff | Zendy; Philipp Hoess | Zendy; Jooske L. Monster | Zendy; Andrea Picco | Zendy; François Nédélec | Zendy; Marko Kaksonen | Zendy; Jonas Ries | Zendy

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Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation

Author(s) -

Markus Mund,

Jan van der Beek,

J.R. Deschamps,

Serge Dmitrieff,

Philipp Hoess,

Jooske L. Monster,

Andrea Picco,

François Nédélec,

Marko Kaksonen,

Jonas Ries

Publication year - 2018

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2018.06.032

Subject(s) - endocytosis , biology , microbiology and biotechnology , actin , nucleation , vesicle , nanotechnology , materials science , cell , genetics , membrane , chemistry , organic chemistry

Clathrin-mediated endocytosis is an essential cellular function in all eukaryotes that is driven by a self-assembled macromolecular machine of over 50 different proteins in tens to hundreds of copies. How these proteins are organized to produce endocytic vesicles with high precision and efficiency is not understood. Here, we developed high-throughput superresolution microscopy to reconstruct the nanoscale structural organization of 23 endocytic proteins from over 100,000 endocytic sites in yeast. We found that proteins assemble by radially ordered recruitment according to function. WASP family proteins form a circular nanoscale template on the membrane to spatially control actin nucleation during vesicle formation. Mathematical modeling of actin polymerization showed that this WASP nano-template optimizes force generation for membrane invagination and substantially increases the efficiency of endocytosis. Such nanoscale pre-patterning of actin nucleation may represent a general design principle for directional force generation in membrane remodeling processes such as during cell migration and division.

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