Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation | Zendy

Tanweer Hussain | Zendy; J.L. Llacer | Zendy; Brian T. Wimberly | Zendy; Jeffrey S. Kieft | Zendy; V. Ramakrishnan | Zendy

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Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation

Author(s) -

Tanweer Hussain,

J.L. Llacer,

Brian T. Wimberly,

Jeffrey S. Kieft,

V. Ramakrishnan

Publication year - 2016

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2016.08.074

Subject(s) - 30s , biology , initiation factor , transfer rna , eif2 , eukaryotic translation , translation (biology) , eukaryotic initiation factor , ribosomal rna , protein subunit , start codon , biophysics , microbiology and biotechnology , messenger rna , biochemistry , rna , gene

In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.

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