Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex | Zendy

Philip J. Robinson | Zendy; Michael J. Trnka | Zendy; David Bushnell | Zendy; Ralph Davis | Zendy; PierreJean Matteï | Zendy; Alma L. Burlingame | Zendy; Roger D. Kornberg | Zendy

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Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex

Author(s) -

Philip J. Robinson,

Michael J. Trnka,

David Bushnell,

Ralph Davis,

PierreJean Matteï,

Alma L. Burlingame,

Roger D. Kornberg

Publication year - 2016

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2016.08.050

Subject(s) - biology , mediator , rna polymerase ii , polymerase , genetics , rna polymerase , microbiology and biotechnology , computational biology , rna , dna , gene , gene expression , promoter

A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.

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