Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides

(Cell 162, 84–95; July 2, 2015) The above article analyzed how Argonaute speeds the search for targets of small silencing RNAs by modifying basic properties of nucleic acid-nucleic acid binding. To compare the thermodynamic and kinetic properties of the Argonaute:RNA guide complex (RISC) to those of nucleic acid hybridization in the absence of the protein, we predicted DG, KD, and koff for nucleic acid alone using nearest-neighbor analysis. Unfortunately, an algebraic error in an Excel spreadsheet used to calculate these values for RNA-RNA interactions led to erroneous predicted values in the text and in Figures 3B, 6A, and S6. These values have been corrected online, and the original and corrected figures appear below. The changes do not alter any of the conclusions drawn from this study; however, the magnitude of some of the reported effects for differential binding upon association of the RNAs within the RISC complex is reduced. We sincerely regret these errors and apologize for any inconvenience that they may have caused.