Single-Cell Transcriptome Analyses Reveal Signals to Activate Dormant Neural Stem Cells | Zendy

Yuping Luo | Zendy; Volkan Coskun | Zendy; Aibing Liang | Zendy; Juehua Yu | Zendy; Liming Cheng | Zendy; Weihong Ge | Zendy; ZhanPing Shi | Zendy; Kunshan Zhang | Zendy; Chun Li | Zendy; Yaru Cui | Zendy; Haijun Lin | Zendy; Dandan Luo | Zendy; Junbang Wang | Zendy; Connie Lin | Zendy; Zachary Dai | Zendy; Hongwen Zhu | Zendy; Jun Zhang | Zendy; Jie Liu | Zendy; Hailiang Liu | Zendy; Jean deVellis | Zendy; Steve Horvath | Zendy; Yi Sun | Zendy; Siguang Li | Zendy

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Single-Cell Transcriptome Analyses Reveal Signals to Activate Dormant Neural Stem Cells

Author(s) -

Yuping Luo,

Volkan Coskun,

Aibing Liang,

Juehua Yu,

Liming Cheng,

Weihong Ge,

ZhanPing Shi,

Kunshan Zhang,

Chun Li,

Yaru Cui,

Haijun Lin,

Dandan Luo,

Junbang Wang,

Connie Lin,

Zachary Dai,

Hongwen Zhu,

Jun Zhang,

Jie Liu,

Hailiang Liu,

Jean deVellis,

Steve Horvath,

Yi Sun,

Siguang Li

Publication year - 2015

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2015.04.001

Subject(s) - biology , ependymal cell , forebrain , neural stem cell , transcriptome , microbiology and biotechnology , fibroblast growth factor , stem cell , lineage (genetic) , gene , neuroscience , receptor , gene expression , genetics , central nervous system

The scarcity of tissue-specific stem cells and the complexity of their surrounding environment have made molecular characterization of these cells particularly challenging. Through single-cell transcriptome and weighted gene co-expression network analysis (WGCNA), we uncovered molecular properties of CD133(+)/GFAP(-) ependymal (E) cells in the adult mouse forebrain neurogenic zone. Surprisingly, prominent hub genes of the gene network unique to ependymal CD133(+)/GFAP(-) quiescent cells were enriched for immune-responsive genes, as well as genes encoding receptors for angiogenic factors. Administration of vascular endothelial growth factor (VEGF) activated CD133(+) ependymal neural stem cells (NSCs), lining not only the lateral but also the fourth ventricles and, together with basic fibroblast growth factor (bFGF), elicited subsequent neural lineage differentiation and migration. This study revealed the existence of dormant ependymal NSCs throughout the ventricular surface of the CNS, as well as signals abundant after injury for their activation.

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