SnapShot: Single-Molecule Fluorescence | Zendy

Susanta K. Sarkar | Zendy; Ambika Bumb | Zendy; Maria Mills | Zendy; Keir C. Neuman | Zendy

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SnapShot: Single-Molecule Fluorescence

Author(s) -

Susanta K. Sarkar,

Ambika Bumb,

Maria Mills,

Keir C. Neuman

Publication year - 2013

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2013.05.024

Subject(s) - biology , snapshot (computer storage) , fluorescence , computational biology , evolutionary biology , computer science , optics , database , physics

Common uorescent agents include organic uorophores (e.g., Cy5), uorescent proteins (e.g., GFP), and semiconductor quantum dots each with different size, photobleaching, blinking, and labeling ef ciency properties. Wideeld detection with EMCCD camera enables parallel measurements. The temporal resolution is >ms, and the working concentration is limited to nM or less. Confocal detection from an effective volume of ~10-15 l with an avalanche photodiode or photomultiplier tube. The temporal resolution is

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