Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer | Zendy

Masahito Tachibana | Zendy; Paula Amato | Zendy; Michelle Sparman | Zendy; Nuria Martí Gutiérrez | Zendy; Rebecca Tippner-Hedges | Zendy; Hong Ma | Zendy; Eunju Kang | Zendy; Alimujiang Fulati | Zendy; Hyosang Lee | Zendy; Hathaitip Sritanaudomchai | Zendy; Keith Masterson | Zendy; Janine M. Larson | Zendy; Deborah Eaton | Zendy; Karen Sadler-Fredd | Zendy; David Battaglia | Zendy; David Lee | Zendy; Diana Wu | Zendy; Jeffrey T. Jensen | Zendy; Phillip E. Patton | Zendy; Sumita Gokhale | Zendy; Richard L. Stouffer | Zendy; Don P. Wolf | Zendy; Shoukhrat Mitalipov | Zendy

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Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer

Author(s) -

Masahito Tachibana,

Paula Amato,

Michelle Sparman,

Nuria Martí Gutiérrez,

Rebecca Tippner-Hedges,

Hong Ma,

Eunju Kang,

Alimujiang Fulati,

Hyosang Lee,

Hathaitip Sritanaudomchai,

Keith Masterson,

Janine M. Larson,

Deborah Eaton,

Karen Sadler-Fredd,

David Battaglia,

David Lee,

Diana Wu,

Jeffrey T. Jensen,

Phillip E. Patton,

Sumita Gokhale,

Richard L. Stouffer,

Don P. Wolf,

Shoukhrat Mitalipov

Publication year - 2013

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2013.05.006

Subject(s) - biology , embryonic stem cell , somatic cell , stem cell , microbiology and biotechnology , somatic cell nuclear transfer , genetics , embryo , embryogenesis , gene , blastocyst

Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state.

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