Single-Cell Dynamics of Genome-Nuclear Lamina Interactions | Zendy

Jop Kind | Zendy; Ludo Pagie | Zendy; Havva Ortabozkoyun | Zendy; Shelagh Boyle | Zendy; Sandra S. de Vries | Zendy; Hans Janssen | Zendy; Mario Amendola | Zendy; Leisha D. Nolen | Zendy; Wendy A. Bickmore | Zendy; Bas van Steensel | Zendy

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Single-Cell Dynamics of Genome-Nuclear Lamina Interactions

Author(s) -

Jop Kind,

Ludo Pagie,

Havva Ortabozkoyun,

Shelagh Boyle,

Sandra S. de Vries,

Hans Janssen,

Mario Amendola,

Leisha D. Nolen,

Wendy A. Bickmore,

Bas van Steensel

Publication year - 2013

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2013.02.028

Subject(s) - biology , epigenetics , genome , mitosis , genetics , cell nucleus , nuclear lamina , evolutionary biology , epigenesis , gene , microbiology and biotechnology , dna methylation , nuclear protein , gene expression , transcription factor

The nuclear lamina (NL) interacts with hundreds of large genomic regions termed lamina associated domains (LADs). The dynamics of these interactions and the relation to epigenetic modifications are poorly understood. We visualized the fate of LADs in single cells using a "molecular contact memory" approach. In each nucleus, only ~30% of LADs are positioned at the periphery; these LADs are in intermittent molecular contact with the NL but remain constrained to the periphery. Upon mitosis, LAD positioning is not detectably inherited but instead is stochastically reshuffled. Contact of individual LADs with the NL is linked to transcriptional repression and H3K9 dimethylation in single cells. Furthermore, we identify the H3K9 methyltransferase G9a as a regulator of NL contacts. Collectively, these results highlight principles of the dynamic spatial architecture of chromosomes in relation to gene regulation.

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