Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells | Zendy

Hui Yang | Zendy; Linyu Shi | Zendy; Bang-An Wang | Zendy; Dan Liang | Zendy; Cuiqing Zhong | Zendy; Wei Liu | Zendy; Yongzhan Nie | Zendy; Jie Liu | Zendy; Jing Zhao | Zendy; Xiang Gao | Zendy; Dangsheng Li | Zendy; Guoliang Xu | Zendy; Jinsong Li | Zendy

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Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells

Author(s) -

Hui Yang,

Linyu Shi,

Bang-An Wang,

Dan Liang,

Cuiqing Zhong,

Wei Liu,

Yongzhan Nie,

Jie Liu,

Jing Zhao,

Xiang Gao,

Dangsheng Li,

Guoliang Xu,

Jinsong Li

Publication year - 2012

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2012.04.002

Subject(s) - biology , ploidy , embryonic stem cell , germline , homologous recombination , oocyte , genetics , stem cell , embryo , zygote , parthenogenesis , gamete , microbiology and biotechnology , homologous chromosome , germ cell , human fertilization , gene , embryogenesis

Haploid cells are amenable for genetic analysis. Recent success in the derivation of mouse haploid embryonic stem cells (haESCs) via parthenogenesis has enabled genetic screening in mammalian cells. However, successful generation of live animals from these haESCs, which is needed to extend the genetic analysis to the organism level, has not been achieved. Here, we report the derivation of haESCs from androgenetic blastocysts. These cells, designated as AG-haESCs, partially maintain paternal imprints, express classical ESC pluripotency markers, and contribute to various tissues, including the germline, upon injection into diploid blastocysts. Strikingly, live mice can be obtained upon injection of AG-haESCs into MII oocytes, and these mice bear haESC-carried genetic traits and develop into fertile adults. Furthermore, gene targeting via homologous recombination is feasible in the AG-haESCs. Our results demonstrate that AG-haESCs can be used as a genetically tractable fertilization agent for the production of live animals via injection into oocytes.

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