SnapShot: Chromosome Conformation Capture

Coverage Commonly < 1Mb Commonly < 1Mb Genome-wide Genome-wide Commonly < 1Mb Genome-wideDetection Locus-specifi c PCR HT-sequencing HT-sequencing HT-sequencing Locus-specifi c qPCR HT-sequencingLimitations Low throughput and coverageLimited coverage Limited to one viewpointRely on one chromatin-associated factor, disregarding other contactsExamples Determine interaction between a known promoter and enhancerDetermine comprehensively higher-order chromosome structure in adefi ned regionAll genes and genomic elements associated with a known LCRAll intra- and interchromosomal associationsDetermine the role of specifi c transcription factors in the interaction between a known promoter and enhancerMap chromatin interaction network of a known transcription factorDerivatives PCR with TaqMan probes