SnapShot: Protein-Protein Interaction Networks | Zendy

J. Seebacher | Zendy; AnneClaude Gavin | Zendy

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SnapShot: Protein-Protein Interaction Networks

Author(s) -

J. Seebacher,

AnneClaude Gavin

Publication year - 2011

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2011.02.025

Subject(s) - biology , snapshot (computer storage) , protein interaction networks , computational biology , protein–protein interaction , microbiology and biotechnology , computer science , database

Methods MethodsYeast two-hybridProtein fragment complementationassayTranscription factor, ubiquitinDehydrofolate reductaseGFP or YFPProtein pairs+ Interacting- NoninteractingInteraction examples- Signaling- Enzyme - substrateInteraction strength-Transient to stableAffinity purification/Mass spectrometryBiochemical purification of affinity-tagged baits followed byMS identification of copurifying preysSplit proteins Assay/Readout

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