O-GlcNAc Transferase Catalyzes Site-Specific Proteolysis of HCF-1 | Zendy

Francesca Capotosti | Zendy; Sophie Guernier | Zendy; Fabienne Lammers | Zendy; Patrice Waridel | Zendy; Yong Cai | Zendy; Jingji Jin | Zendy; Joan Conaway | Zendy; Ronald Conaway | Zendy; Winship Herr | Zendy

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O-GlcNAc Transferase Catalyzes Site-Specific Proteolysis of HCF-1

Author(s) -

Francesca Capotosti,

Sophie Guernier,

Fabienne Lammers,

Patrice Waridel,

Yong Cai,

Jingji Jin,

Joan Conaway,

Ronald Conaway,

Winship Herr

Publication year - 2011

Publication title -

cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 26.304

H-Index - 776

eISSN - 1097-4172

pISSN - 0092-8674

DOI - 10.1016/j.cell.2010.12.030

Subject(s) - proteolysis , biology , protein subunit , transferase , biochemistry , microbiology and biotechnology , cleavage (geology) , cell cycle , enzyme , cell , gene , paleontology , fracture (geology)

The human epigenetic cell-cycle regulator HCF-1 undergoes an unusual proteolytic maturation process resulting in stably associated HCF-1(N) and HCF-1(C) subunits that regulate different aspects of the cell cycle. Proteolysis occurs at six centrally located HCF-1(PRO)-repeat sequences and is important for activation of HCF-1(C)-subunit functions in M phase progression. We show here that the HCF-1(PRO) repeat is recognized by O-linked β-N-acetylglucosamine transferase (OGT), which both O-GlcNAcylates the HCF-1(N) subunit and directly cleaves the HCF-1(PRO) repeat. Replacement of the HCF-1(PRO) repeats by a heterologous proteolytic cleavage signal promotes HCF-1 proteolysis but fails to activate HCF-1(C)-subunit M phase functions. These results reveal an unexpected role of OGT in HCF-1 proteolytic maturation and an unforeseen nexus between OGT-directed O-GlcNAcylation and proteolytic maturation in HCF-1 cell-cycle regulation.

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