Open AccessAqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity
Author(s) -
Ana Carolina Silveira Rabêlo,
Glaucy Rodrigues de Araújo,
Karine de Pádua Lúcio,
Carolina Morais Araújo,
Pedro Henrique de Amorim Miranda,
Breno de M. Silva,
Ana Cláudia Alvarenga Carneiro,
Érica Milena de Castro Ribeiro,
Wanderson Geraldo de Lima,
Gustavo Henrique Bianco de Souza,
Geraldo Célio Brandão,
Daniela Caldeira Costa
Publication year - 2017
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2017.09.003
Subject(s) - chemistry , antioxidant , reactive oxygen species , oxidative stress , nitric oxide , lipid peroxidation , ethanol , biochemistry , glutathione , oxygen radical absorbance capacity , pharmacology , dpph , biology , enzyme , organic chemistry
The metabolism of ethanol occurs mainly in the liver, promoting increase of reactive oxygen species and nitrogen, leading to redox imbalance. Therefore, antioxidants can be seen as an alternative to reestablish the oxidizing/reducing equilibrium. The aim of this study was to evaluate the antioxidant and hepatoprotective effect of aqueous extract of Baccharis trimera (Less.) DC., Asteraceae, in a model of hepatotoxicity induced by ethanol. The extract was characterized and in vitro tests were conducted in HepG2 cells. It was evaluated the cells viability exposed to aqueous extract for 24 h, ability to scavenging the radical DPPH, besides the production of reactive oxygen species and nitric oxide, and the influence on the transcriptional activity of transcription factor Nrf2 (12 and 24 h) after exposure to 200 mM ethanol. The results showed that aqueous extract was non-cytotoxic in any concentration tested; moreover, it was observed a decrease in ROS and NO production, also promoting the transcriptional activity of Nrf2. In vivo, we pretreatment male rats Fisher with 600 mg/kg of aqueous extract and 1 h later 5 ml/kg of absolute ethanol was administrated. After two days of treatment, the animals were euthanized and lipid profile, hepatic and renal functions, antioxidant status and oxidative damage were evaluated. The treatment with extract improved liver function and lipid profile, reflecting the reduction of lipid microvesicules in the liver. It also promoted an increase of glutathione peroxidase activity, decrease of oxidative damage and MMP-2 activity. These results, analyzed together, suggest the hepatoprotective effect of B. trimera aqueous extract