Open AccessQuantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus
Author(s) -
Leonardo C. Silva,
Rúbia Darc Machado,
Dayane R. Silva,
Vanessa Cristiane Santana Amaral,
José Realino de Paula,
Edemilson Cardoso da Conceição,
Joelma Abadia Marciano de Paula
Publication year - 2017
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2017.07.002
Subject(s) - catechin , chromatography , chemotype , citral , chemistry , relative standard deviation , linear range , gradient elution , high performance liquid chromatography , detection limit , polyphenol , organic chemistry , antioxidant , essential oil
This paper describes the quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus (Gomes) Landrum, Myrtaceae, citral chemotype using a validated HPLC-PDA method. The method employs a RP-18 column with acetonitrile:water-orthophosphoric acid 0.05% (gradient system) and UV detection at 210 nm. The method was demonstrated to be simple, sensitive, specific, linear, precise, accurate and robust. The response was linear over a range of 5–200 µg/ml (r > 0.999). The range of recoveries was 92.27–102.54%. The relative standard deviation values for intra- and inter-day precision studies were 4.30 and 3.78%, respectively. This assay can be readily utilized as quality control method for catechin in the dried extract of P. pseudocaryophyllus