Open AccessPhytochemical study of Pilosocereus pachycladus and antibiotic-resistance modifying activity of syringaldehyde
Author(s) -
Severino Gonçalves de Brito-Filho,
Jéssica Karina da Silva Maciel,
Yanna Carolina Ferreira Teles,
Milen Maria Magalhães de Souza Fernandes,
Otemberg Souza Chaves,
Maria Denise Leite Ferreira,
Pedro Dantas Fernandes,
Leonardo P. Félix,
Isis Caroline da Silva Cirino,
José P. Siqueira-Júnior,
Raimundo Braz-Filho,
María de Fátima Vanderlei de Souza
Publication year - 2017
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2017.06.001
Subject(s) - syringaldehyde , chemistry , efflux , antibiotics , minimum inhibitory concentration , bacteria , microbiology and biotechnology , traditional medicine , biochemistry , biology , medicine , vanillin , genetics
Pilosocereus pachycladus F. Ritter, Cactaceae, popularly known as "facheiro", is used as food and traditional medicine in Brazilian caatinga ecoregion. The plant is used to treat prostate inflammation and urinary infection. The present work reports the first secondary metabolites isolated from P. pachycladus. Therefore, the isolated compound 4-hydroxy-3,5-dimethoxy benzaldehyde (syringaldehyde) was evaluated as modulator of Staphylococcus aureus pump efflux-mediated antibiotic resistance. The isolation of compounds was performed using chromatographic techniques and the structural elucidation was carried out by spectroscopic methods. In order to evaluate syringaldehyde ability to modulate S. aureus antibiotic resistance, its minimum inhibitory concentrations (µg/ml) was first determinate, then, the tested antibiotics minimum inhibitory concentrations were determined in the presence of the syringaldehyde in a sub-inhibitory concentration. The chromatographic procedures led to isolation of twelve compounds from P. pachycladus including fatty acids, steroids, chlorophyll derivatives, phenolics and a lignan. The syringaldehyde did not show any antibacterial activity at 256 µg/ml against S. aureus. On the other hand the compound was able to reduce the antibiotic concentration (tetracycline, norfloxacin, ethidium bromide) required to inhibit the growth of drug-resistant bacteria, showing the ability of syringaldehyde of inhibiting the efflux pump on these bacteria