Open AccessToxicity and antitumor efficacy of Croton polyandrus oil against Ehrlich ascites carcinoma cells
Author(s) -
Déborah Ribeiro Pessoa Meireles,
Heloísa Fernandes,
Thaísa Leite Rolim,
Tatianne Mota Batista,
Vivianne Mendes Mangueira,
Tatyanna Kélvia Gomes de Sousa,
João Carlos Lima Rodrigues Pita,
Aline Lira Xavier,
Daiene Martins Beltrão,
Josean Fechine Tavares,
Marcelo S. Silva,
Karina K.P. Medeiros,
Marianna Vieira Sobral
Publication year - 2016
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2016.05.014
Subject(s) - toxicity , leukopenia , pharmacology , ehrlich ascites carcinoma , micronucleus test , hemolysis , micronucleus , lymphocytopenia , acute toxicity , genotoxicity , croton oil , essential oil , biology , in vivo , chemistry , medicine , immunology , lymphocyte , inflammation , botany , microbiology and biotechnology
The essential oil from Croton polyandrus Spreng., Euphorbiaceae, leaves was tested for the toxicity and antitumor activity. The concentration producing 50% hemolysis was 141μg/ml on mice erythrocytes. In the acute toxicological study, the estimated LD50 was 447.18mg/kg. The essential oil did not induce increase in number of micronucleated erythrocytes, suggesting low genotoxicity. Essential oil (100 or 150mg/kg) showed significant antitumor activity in Ehrlich ascitic carcinoma model. We observed that essential oil induces cell-cycle arrest at the G0/G1 phase, and increases the sub-G1 peak, which represents a marker of cell death by apoptosis. Survival also increased for the treated animals. The toxicological analyses revealed reduction in body weight, increased aspartate aminotransferase and alanine aminotransferase activity, hematological changes, and a thymus index reduction. These data suggest gastrointestinal and liver toxicity, anemia, leukopenia/lymphocytopenia, and immunosuppressive effects. Histopathological analysis revealed the weak hepatotoxicity of essential oil. In summary, essential oil of C. polyandrus displays in vivo antitumor activity and moderate toxicity