Open AccessAmazon emulsions as cavity cleansers: antibacterial activity, cytotoxicity and changes in human tooth color
Author(s) -
Cristiane Coelho De Bari,
Fábio Correia Sampaio,
Nikeila C. O. Conde,
Luanny Gomes Moura,
Valdir Veiga Júnior,
Gleyce dos Santos Barbosa,
Marne Carvalho de Vasconcellos,
Carina Toda,
Gisely Naura Venâncio,
Maria Fulgência Costa Lima Bandeira
Publication year - 2016
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2016.03.010
Subject(s) - emulsion , chemistry , cytotoxicity , antibacterial activity , streptococcus mutans , minimum inhibitory concentration , streptococcus salivarius , antimicrobial , minimum bactericidal concentration , viability assay , food science , microbiology and biotechnology , bacteria , biology , biochemistry , cell , in vitro , organic chemistry , genetics
The copaiba oleoresin, Copaifera multijuga Hayne, Fabaceae, is a phytotherapeutic agent with antimicrobial activity. This study evaluated the antibacterial activity and cytotoxicity of, and tooth color changes caused by four copaiba oil emulsions (Emulsion 1, 10% CM; Emulsion 2, 10% C. multijuga+1% biotech product; Emulsion 3, 30% C. multijuga; and Emulsion 4, 30% C. multijuga+1% biotech product). The antibacterial activities against microorganisms causing dental caries (Streptococcus mutans ATCC25175, S. oralis ATCC10557, S. salivarius ATCC7073, and Lactobacillus casei ATCC7469) were tested using three parameters: minimum inhibitory concentration, minimum bactericidal concentration, and cell viability by fluorescence microscopy. The emulsions were assessed for cytotoxicity by means of the hemolytic assay and cell culture (murine fibroblast cells NHI3T3) using Alamar Blue™. The dentin color change caused by the emulsions was examined at 10s, 30s, and 10min. The emulsions showed antibacterial activity against the microorganisms tested with an MIC of 125μl/ml. The minimum bactericidal concentration was higher than minimum inhibitory concentration for the tested microorganism and the fluorescence confirmed that the cells were viable at minimum inhibitory concentration values. The emulsions had a hemolytic activity of 71.16% (Emulsion 3) and 44.67% (Emulsion 4) at a concentration of 30μl/ml. In cell culture assay, NHI-3T3 cells treated with the emulsions showed 6–16% viability. Emulsion 1 caused clinically imperceptible color change in dentin at 10s (ΔE=3.21), Emulsion 2 at 30s (ΔE=2.70) and 10min (ΔE=3.08), and Emulsion 4 at 10min (ΔE=3.03). Emulsion 3 caused color change at all times tested. This research documented positive data regarding antibacterial activity, cytotoxicity, and tooth color changes when using copaiba oleoresin emulsions, showing its potential for use in dentistry